p erk (R&D Systems)
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p erk
P Erk, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+erk1+erk2/Human%2FMouse%2FRat+Phospho-ERK1+(T202%2FY204)%2FERK2+(T185%2FY187)+Antibody/pm41812248-49-52-65
Average 93 stars, based on 36 article reviews
P Erk, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+erk1+erk2/Human%2FMouse%2FRat+Phospho-ERK1+(T202%2FY204)%2FERK2+(T185%2FY187)+Antibody/pm41812248-49-52-65
Average 93 stars, based on 36 article reviews
p erk - by Bioz Stars,
2026-10
93/100 stars
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Incubation:Article Title: Hsp90 Inhibition Overcomes HGF-Triggering Resistance to EGFR-TKIs in EGFR-Mutant Lung Cancer by Decreasing Client Protein Expression and Angiogenesis Article Snippet: Protein aliquots of 25 μg each were resolved by sodium dodecyl sulfate polyacrylamide gel (Bio-Rad, Hercules, CA) electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After washing four times, the membranes were incubated with Blocking One (Nacalai Tesque, Inc., Kyoto, Japan) for 1 hour at room temperature and overnight at 4°C with primary antibodies to β-actin (13E5), MET (25H2), phospho-Met (anti-p-Met, Y1234/ Y1235; 3D7), p-EGFR (Y1068), Akt or p-Akt (S473) (Cell Signaling Technology, Beverly, MA), human EGFR (1 μg/ml), human/mouse/rat ERK1/ERK2 (0.2 μg/ml), and Article Title: Ability of the Met Kinase Inhibitor Crizotinib and New Generation EGFR Inhibitors to Overcome Resistance to EGFR Inhibitors Article Snippet: Protein aliquots of 25 μg each were resolved by SDS polyacrylamide gel (Bio-Rad, Hercules, CA) electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After washing 3 times, the membranes were incubated with Blocking One (Nacalai Tesque, Inc., Kyoto, Japan) for 1 hour at room temperature and then incubated overnight at 4°C with primary antibodies to Met (25H2), phospho-Met (anti-p-Met, Y1234/Y1235; 3D7), p-EGFR (Y1068), Akt, p-Akt (S473) (Cell Signaling Technology, Beverly, MA); human EGFR (1 μg/mL), human/mouse/rat ERK1/ERK2 (0.2 μg/mL), and Article Title: mTOR Inhibitors Control the Growth of EGFR Mutant Lung Cancer Even after Acquiring Resistance by HGF Article Snippet: Protein aliquots (25 μg each) were resolved by SDS polyacrylamide gel electrophoresis (Bio-Rad, Hercules, CA) and transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After washing 4 times, the membranes were incubated with Blocking One (Nacalai Tesque, Inc., Kyoto, Japan) for 1 h at room temperature (RT) and overnight at 4°C with primary antibodies to β-actin (13E5), Met (25H2), phospho-MET (anti-p-MET, Y1234/Y1235; 3D7), p-EGFR (Y1068), AKT or p-AKT (S473), mTOR, p-mTOR (S2448), p70S6K, pp70S6K (T389), 4E-BP1, p4E-BP1(T37/46) (Cell Signaling Technology, Beverly, MA), human EGFR (1 μg/mL), human/mouse/rat ERK1/ERK2 (0.2 μg/mL), and Article Title: Triple Inhibition of EGFR, Met, and VEGF Suppresses Regrowth of HGF-Triggered, Erlotinib-Resistant Lung Cancer Harboring an EGFR Mutation Article Snippet: Protein aliquots of 25 μg each were resolved by sodium dodecyl sulfate-polyacrylamide gel (Bio-Rad, Hercules, CA) electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After washing four times, the membranes were incubated with Blocking One (Nacalai Tesque, Kyoto, Japan) for 1 hour at room temperature and overnight at 4°C with primary antibodies to β-actin (13E5), Met (25H2), phospho-Met (Y1234/Y1235;3D7), phospho-EGFR (Y1068), Akt, phospho-Akt (Ser473; 736E11), VEGFR-2 (55B11), phospho-VEGFR-2 (Tyr951;15D2), human EGFR (1 μg/ml), human/mouse/rat Erk1/Erk2 (0.2 μg/ml), and Article Title: Triple Inhibition of EGFR, Met, and VEGF Suppresses Regrowth of HGF-Triggered, Erlotinib-Resistant Lung Cancer Harboring an EGFR Mutation Article Snippet: Protein aliquots of 25 μg each were resolved by sodium dodecyl sulfate-polyacrylamide gel (Bio-Rad, Hercules, CA) electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After washing four times, the membranes were incubated with Blocking One (Nacalai Tesque, Kyoto, Japan) for 1 hour at room temperature and overnight at 4°C with primary antibodies to β-actin (13E5), Met (25H2), phospho-Met (Y1234/Y1235;3D7), phospho-EGFR (Y1068), Akt, phospho-Akt (Ser473; 736E11), VEGFR-2 (55B11), phospho-VEGFR-2 (Tyr951;15D2), human EGFR (1 μg/ml), human/mouse/rat Erk1/Erk2 (0.2 μg/ml), and Blocking Assay:Article Title: Hsp90 Inhibition Overcomes HGF-Triggering Resistance to EGFR-TKIs in EGFR-Mutant Lung Cancer by Decreasing Client Protein Expression and Angiogenesis Article Snippet: Protein aliquots of 25 μg each were resolved by sodium dodecyl sulfate polyacrylamide gel (Bio-Rad, Hercules, CA) electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After washing four times, the membranes were incubated with Blocking One (Nacalai Tesque, Inc., Kyoto, Japan) for 1 hour at room temperature and overnight at 4°C with primary antibodies to β-actin (13E5), MET (25H2), phospho-Met (anti-p-Met, Y1234/ Y1235; 3D7), p-EGFR (Y1068), Akt or p-Akt (S473) (Cell Signaling Technology, Beverly, MA), human EGFR (1 μg/ml), human/mouse/rat ERK1/ERK2 (0.2 μg/ml), and Article Title: Ability of the Met Kinase Inhibitor Crizotinib and New Generation EGFR Inhibitors to Overcome Resistance to EGFR Inhibitors Article Snippet: Protein aliquots of 25 μg each were resolved by SDS polyacrylamide gel (Bio-Rad, Hercules, CA) electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After washing 3 times, the membranes were incubated with Blocking One (Nacalai Tesque, Inc., Kyoto, Japan) for 1 hour at room temperature and then incubated overnight at 4°C with primary antibodies to Met (25H2), phospho-Met (anti-p-Met, Y1234/Y1235; 3D7), p-EGFR (Y1068), Akt, p-Akt (S473) (Cell Signaling Technology, Beverly, MA); human EGFR (1 μg/mL), human/mouse/rat ERK1/ERK2 (0.2 μg/mL), and Article Title: mTOR Inhibitors Control the Growth of EGFR Mutant Lung Cancer Even after Acquiring Resistance by HGF Article Snippet: Protein aliquots (25 μg each) were resolved by SDS polyacrylamide gel electrophoresis (Bio-Rad, Hercules, CA) and transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After washing 4 times, the membranes were incubated with Blocking One (Nacalai Tesque, Inc., Kyoto, Japan) for 1 h at room temperature (RT) and overnight at 4°C with primary antibodies to β-actin (13E5), Met (25H2), phospho-MET (anti-p-MET, Y1234/Y1235; 3D7), p-EGFR (Y1068), AKT or p-AKT (S473), mTOR, p-mTOR (S2448), p70S6K, pp70S6K (T389), 4E-BP1, p4E-BP1(T37/46) (Cell Signaling Technology, Beverly, MA), human EGFR (1 μg/mL), human/mouse/rat ERK1/ERK2 (0.2 μg/mL), and Article Title: Triple Inhibition of EGFR, Met, and VEGF Suppresses Regrowth of HGF-Triggered, Erlotinib-Resistant Lung Cancer Harboring an EGFR Mutation Article Snippet: Protein aliquots of 25 μg each were resolved by sodium dodecyl sulfate-polyacrylamide gel (Bio-Rad, Hercules, CA) electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After washing four times, the membranes were incubated with Blocking One (Nacalai Tesque, Kyoto, Japan) for 1 hour at room temperature and overnight at 4°C with primary antibodies to β-actin (13E5), Met (25H2), phospho-Met (Y1234/Y1235;3D7), phospho-EGFR (Y1068), Akt, phospho-Akt (Ser473; 736E11), VEGFR-2 (55B11), phospho-VEGFR-2 (Tyr951;15D2), human EGFR (1 μg/ml), human/mouse/rat Erk1/Erk2 (0.2 μg/ml), and Article Title: Triple Inhibition of EGFR, Met, and VEGF Suppresses Regrowth of HGF-Triggered, Erlotinib-Resistant Lung Cancer Harboring an EGFR Mutation Article Snippet: Protein aliquots of 25 μg each were resolved by sodium dodecyl sulfate-polyacrylamide gel (Bio-Rad, Hercules, CA) electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad). .. After washing four times, the membranes were incubated with Blocking One (Nacalai Tesque, Kyoto, Japan) for 1 hour at room temperature and overnight at 4°C with primary antibodies to β-actin (13E5), Met (25H2), phospho-Met (Y1234/Y1235;3D7), phospho-EGFR (Y1068), Akt, phospho-Akt (Ser473; 736E11), VEGFR-2 (55B11), phospho-VEGFR-2 (Tyr951;15D2), human EGFR (1 μg/ml), human/mouse/rat Erk1/Erk2 (0.2 μg/ml), and |